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IDENTIFICATION OF INTESTINAL MICROBES IN CHILDREN WITH DIARRHEA ANDNON-DIARRHEA USING POLYMERASE CHAIN REACTION / ELECTROSPRAY IONIZATION-MASS SPECTROMETRY (PCR / ESI-MS)
Abstract
Abstract :Microbiota present in human intestinal are diverse, and imbalance in composition of intestinal flora may cause diarrhea.This study aimed to obtain a profile of intestinal bacteria in children with and without diarrhea and assess their presence with incidence of diarrhea. An analitical descriptive with cross sectional design study was carried out. A stool specimen was collected from each children of 2-12 years old with and without diarrhea who lived in North Jakarta. DNA extraction was performed prior to detection of microbes using Polymerase Chain Ceaction/Electrospray Ionization-Mass Spectrometry.
Eighty stool specimens consisted of 33 and 47 specimens from children with and without diarrhea were included in the study. Thirty single and 6 multiple matches were detected in 30 specimens of the diarrhea group; 28 single and 8 multiple matches were found in 34 specimens of the non-diarrhea.Escherechiacoli and Klebsiella pneumonia were predominant in both groups. Firmicutes, Proteobacteria and Bacteroidetes were deteced in the diarrhea group, while Actinobacteria, Proteobacteria and Verrucomicrobia were in the non-diarrhea. The relationship of incidence of diarrhea and the present of enteropathogens in the stool was not significant, however, there was a strong correlation of the risk of suffering diarrhea due to the presence of enteropathogens (OR = 0.724 with 95%, CI: 0.237-2.215).
In conclusion, most bacteria detected in both groups were similar, nonetheless, Actinobacteria was present only in the non-diarrhea. The chance to have diarrhea was higher when enteropathogen was detected in the stool.
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Sekirov I, Russell SL, Antunes LC, Finlay BB. Gut microbiota in health and disease. Physiol Rev 2010;90:859-904
Ley RE, Peterson DA, Gordon JI. Ecological and evolutionary forces shaping microbial diversity in the human intestine. Cell 2006;124:837-48
Sommer F, Backhed F. The gut microbiota--masters of host development and physiology. Nat Rev Microbiol 2013;11:227-38.
Frank DN, St Amand AL, Feldman RA, Boedeker EC, Harpaz N, Pace NR.Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. Proc Natl AcadSci U S A 2007;104:13780-5
Lupp C, Robertson ML, Wickham ME, Sekirov I, Champion OL, Gaynor EC, et al. Host-mediated inflammation disrupts the intestinal microbiota and promotes the overgrowth of Enterobacteriaceae. Cell Host Microbe 2007;2:204.
Eckburg P, Bik E, Bernstein C, Purdom E, Dethlefsen L, Sargent M, et al. Diversity of the human intestinal microbial flora. Science 2005:1635-8.
Zoetendal EG, von Wright A, Vilpponen-Salmela T, Ben-Amor K, Akkermans AD, de Vos WM. Mucosa-associated bacteria in the human gastrointestinal tract are uniformly distributed along the colon and differ from the community recovered from feces. Appl Environ Microbiol 2002;68:3401-7.
Franks AH, Harmsen HJ, Raangs GC, Jansen GJ, Schut F, Welling GW. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl Environ Microbiol 1998;64:3336-459. Kaleta EJ, Clark AE, Johnson DR, Gamage DC, Wysocki VH, Cherkaoui A, et al. Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles. J ClinMicrobiol 2011;49:345-53.
Ecker DJ, Sampath R, Blyn LB, Eshoo MW, Ivy C, Ecker JA, et al. Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance. Proc Natl AcadSci U S A 2005;102:8012-7.
Brinkman CL, Vergidis P, Uhl JR, Pritt BS, Cockerill FR, Steckelberg JM, et al. PCR-electrospray ionization mass spectrometry for direct detection of pathogens and antimicrobial resistance from heart valves in patients with infective endocarditis. J Clin Microbiol 2013;51:2040-6.
Jacob D, Sauer U, Housley R, Washington C, Sannes-Lowery K, Ecker DJ, et al. Rapid and high-throughput detection of highly pathogenic bacteria by Ibis PLEX-ID technology. PLoS One 2012;7:e39928
WHO. Diarrhoeal disease. In: World Health Organization Fact Sheet 2009.
Inc AM. PLEX-ID System Customer Training Guide. In; 2011.
Oyofo B, Subekti D, Tjaniadi P, Machpud N, Komalarini S, Setiawan B. Enteropathogen associated with acute diarrhea in community and hospital patients in Jakarta, Indonesia. FEMS Immun and Med Microbiol 2002:139-46.
Kementerian Kesehatan RI. Situasi diare di Indonesia. Buletin Jendela Data &Informasi Kesehatan; 2011.
Sampath R, Mulholland N, Blyn LB, Massire C, Whitehouse CA, Waybright N, et al. Comprehensive biothreat cluster identification by PCR/electrospray-ionization mass spectrometry. PLoS One 2012;7:e36528
Rost M. PLEX-ID enquiries from Indonesia. In: Meulila F, ed.; 2013.
Bannister B, Gillespie S, Jones J. Infection Microbiology and Management 3rd ed. Massachusetts: Blackwell Publishing; 2006.
Bannister B, Gillespie S, Jones J. Infection Microbiology and Management 3rd ed. Massachusetts: Blackwell Publishing; 2006.
Pawlowski D, Raslawsky A, Siebert G, Metzger D, Koudelka G, Karalus R. Identification of Hylemonella gracilis as an Antagonist of Yersinia pestis Persistence J BioterrBiodef S3:004 2011:doi:10.4172/2157-526.
Malkan AD, Strollo W, Scholand SJ, Dudrick SJ. Implanted-port-catheter-related sepsis caused by Acidovorax avenae and methicillin-sensitive Staphylococcus aureus. J ClinMicrobiol 2009;47:3358-61.
Everard A, Belzer C, Geurts L, Ouwerkerk JP, Druart C, Bindels LB, et al. Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity. Proc Natl AcadSci U S A 2013;110:9066-71.
Buffie CG, Pamer EG. Microbiota-mediated colonization resistance against intestinal pathogens. Nature reviews Immunology 2013;13:790-801.
Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, et al. Human gut microbiome viewed across age and geography. Nature 2012;486:222-7.
Monstein HJ, Tiveljung A, Kraft CH, Borch K, Jonasson J. Profiling of bacterial flora in gastric biopsies from patients with Helicobacter pylori-associated gastritis and histologically normal control individuals by temperature gradient gel electrophoresis and 16S rDNAsequence analysis. J Med Microbiol 2000;49:817-22.
Marteau P, Pochart P, Dore J, Bera-Maillet C, Bernalier A, Corthier G. Comparative study of bacterial groups within the human cecal and fecal microbiota. Appl Environ Microbiol 2001;67:4939-42.
Buktiwetan P, Surjawidjaja J, Salim O, Aidilifit M, Lesmana M. Diarebakterial : etiologi dan kepekaan Antibiotika di
dua Pusat Kesehatan Masyarakat di Jakarta. Jkedokter Trisakti 2001;20:57-65.
Baumgardner DJ. Soil-related bacterial and fungal infections. Journal of the American Board of Family Medicine:JABFM 2012;25:734-44.
Haagsma J. Pathogenic anaerobic bacteria and the environment. Rev Sci Tech 1991;10:749-64.
DOI: https://doi.org/10.32667/ijid.v2i2.21
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