Ekstrak Moringa oleifera Sebagai Antiseptik Terhadap Escherichia coli dan Streptococcus pyogenes Menggunakan Metode Percentage Kill
Downloads
Latar belakang: Kandungan kimia daun kelor (M.oleifera) menunjukan sifat antibakteri terhadap bakteri Gram positif dan Gram negatif dengan menghambat sintesis dan metabolisme DNA serta menghancurkan dinding sel. Penelitian ini bertujuan untuk mengetahui signifikansi kemampuan ekstrak daun M.oleifera sebagai antiseptik terhadap E. coli dan S. pyogenes. Metode: Uji percentage kill dilakukan untuk mengetahui persentase kematian bakteri setelah kontak dengan M. oleifera pada menit ke 1, 2 dan 5. Variabel yang digunakan yaitu kontrol dan perlakuan yang dilakukan secara bersamaan. Uji dinyatakan memenuhi standar bila memberikan hasil ≥90% untuk setiap waktu kontak. Hasil: Uji terhadap E. coli pada menit ke 1, 2 dan 5 menunjukkan hasil masing-masing 93.41%, 94.14%, dan 96.87%, sedangkan terhadap S. pyogenes masing-masing 73.27%, 83.15% dan 94.19%. Terhadap S. pyogenes pada menit ke-5 memenuhi standar. Kesimpulan: M. oleifera efektif mengeliminasi E. coli karena nilai percentage kill untuk semua waktu kontak ≥90% dengan nilai tertinggi pada menit ke-5 (96.87%), sedangkan terhadap S. pyogenes ekstrak daun M. oleifera menunjukkan aktifitas mikroba yang baik pada waktu kontak 5 menit (94.19%).
Downloads
Conceição RA, Ludovico MS, Andrade C, Yano T. Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture. Brazilian J Med Biol Res. 2012;45:417–24.
Polat G, Ugan RA, Cadirci E, Halici Z. Sepsis and septic shock: current treatment strategies and new approaches. Eurasian J Med. 2017;49(1):53.
Gyawali B, Ramakrishna K, Dhamoon AS. Sepsis: The evolution in definition, pathophysiology, and management. SAGE open Med. 2019;7:2050312119835043.
World Health Organization archives. Disinfectants and antiseptics [Internet]. Geneva: World Health Organization; 2004 [cited 2021 Jan 22]. Available from: http://archives.who.int/eml/wmf/2004/English/Disinfectants and antiseptics.pdf.
Abdull Razis AF, Ibrahim MD, Kntayya SB. Health benefits of Moringa oleifera. Asian pacific J cancer Prev. 2014;15(20):8571–6.
Kou X, Li B, Olayanju JB, Drake JM, Chen N. Nutraceutical or pharmacological potential of Moringa oleifera Lam. Nutrients. 2018;10(3):343.
Brugger SD, Baumberger C, Jost M, Jenni W, Brugger U, Mühlemann K. Automated counting of bacterial colony forming units on agar plates. PLoS One. 2012;7(3):e33695.
Conny RT. Operational Procedure : Percentage kill. Microbiology Clinical Laboratory. Jakarta: Universitas Indonesia; 2022.
Jan BS ASTM E2315. Standard Guide for Assessment of Microbial Activity Using a Time-Kill Procedure. Texas: Microchem Laboratory; 2019.
Farmakope Indonesia. Ministry of Health of The Republic Indonesia, 6th edition. Jakarta; 2020.
Raja RR, Sreenivasulu M, Vaishnavi S, Navyasri DM, Samatha G, Geethalakshmi S. Moringa oleifera-An overview. RA J Appl Res. 2016;2(9):620–4.
Abdalla AM, Alwasilah HY, Mahjoub RAH, Mohammed HI, Yagoub M. Evaluation of antimicrobial activity of Moringa oleifera leaf extracts against pathogenic bacteria isolated from urinary tract infected patients. J Adv Lab Res Biol. 2016;7(2):47–51.
Russell AD. Mechanisms of antimicrobial action of antiseptics and disinfectants: an increasingly important area of investigation. J Antimicrob Chemother. 2002;49(4):597–9.
Abubakar I, Usman A. Phytochemical and antibacterial investigations of moringa (Moringa oleifera) leaf extract on selected bacterial pathogens. J Microbiol Antimicrob. 2016;8(5):28–33.
Unegbu V, Nkwoemeka N, Okey-Ndeche F, Obum-Nnadi C. Phytochemical and Antibacterial Properties of Moringa oleifera leaf extracts on Escherichia coli and Staphylococcus aureus. Niger J Microbiol. 2020;34(1):5145–52.
Bouarab-Chibane L, Forquet V, Lantéri P, Clément Y, Léonard-Akkari L, Oulahal N, et al. Antibacterial properties of polyphenols: characterization and QSAR (Quantitative structure–activity relationship) models. Front Microbiol. 2019;10:829.
Kaczmarek B. Tannic acid with antiviral and antibacterial activity as a promising component of biomaterials—A minireview. Materials (Basel). 2020;13 (14):3224.
Dima LRH. Uji aktivitas antibakteri ekstrak daun kelor (Moringa oleifera L.) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Pharmacon. 2016;5(2).
Rachmawati SR. Characterization Of Moringa (Moringa Oleifera Lam.) Leaf Water Extracts By Chemical And Microbiology. SANITAS J Teknol Dan Seni Kesehat. 2019;10(2):102–16.
Gopalakrishnan L, Doriya K, Kumar DS. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Sci Hum wellness. 2016;5(2):49–56.
Brilhante RSN, Sales JA, Pereira VS, Castelo D de SCM, de Aguiar Cordeiro R, de Souza Sampaio CM, et al. Research advances on the multiple uses of Moringa oleifera: A sustainable alternative for socially neglected population. Asian Pac J Trop Med. 2017;10(7):621–30.
Moyo B, Masika PJ, Muchenje V. Antimicrobial activities of Moringa oleifera Lam leaf extracts. African J Biotechnol. 2012;11(11):2797–802.
Oluduro AO. Evaluation of antimicrobial properties and nutritional potentials of Moringa oleifera Lam. leaf in South-Western Nigeria. Malays J Microbiol. 2012;8(2):59–67.
Floorentina D. Pengaruh konsentrasi ekstrak daun kelor terhadap pertumbuhan Bakteri Streptococcus pyogenes pada plat resin akrilik aktivasi [Internet]. Yogyakarta: UNY; 2015. Available from: http://repository.umy.ac.id/ handle/123456789/18167
